Distance grown along leaf petioles and <i>Leptosphaeria maculans</i> DNA in petioles and stems.

<p>Leaf laminas or leaf petioles of doubled haploid lines A30 (susceptible) or C119 (with quantitative resistance) were inoculated with conidia of GFP-expressing <i>L. maculans</i> isolate ME24/3.13 or ascospores from a natural population. The distance grown by <i>L. maculans</i> along the leaf vein/petiole towards the stem (leaf lamina inoculation, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0084924#pone-0084924-g002" target="_blank">Fig. 2A</a>) was measured by assessing extent of GFP fluorescence at 22–25 days post inoculation (dpi) for conidial inoculation (A) or by assessing extent of the visible necrosis (A) and by quantification of amount of <i>L. maculans</i> DNA (B) using qPCR at 18–20 dpi for ascospore inoculation. The growth of <i>L. maculans</i> in stem tissue (petiole inoculation, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0084924#pone-0084924-g002" target="_blank">Fig. 2B</a>) was assessed by quantification of amount of <i>L. maculans</i> DNA using qPCR (B) at 46 dpi inoculation with ascospores. Bars show standard errors (A, 28 d.f. for conidia and 54 d.f. for ascospores; B, 54 d.f. for leaf petiole and 50 d.f. for stem).