Comparison of amino acid sequences between the softener and other known proteins by BLAST.

<p>In N-terminal amino acid sequence of the softener, third X residue means unidentified residue and 14th X residue means proline or phenylalanine. The third residue was excluded in calculation of the identity. Identical residues were boxed. (A) Sequence comparison between the softener and partial amino acid sequence of ubiquitin carboxyl-terminal hydrolase 34-like protein from <i>Strongylocentrotus purpuratus</i> (XP_785288.3) that showed the highest bit score among echinoderms. Identity was 47.1%. (B) Sequence comparison to partial amino acid sequence of GntR family transcriptional regulator from <i>Carnobacterium</i> sp. 17-4 (YP_004375176.1) that showed the highest bit score among whole organism. Identity was 56.3%.