miR-24 inhibits CHOP-induced Bim overexpression in ER mediated apoptosis pathway.

<p>(A) Validation of knockdown efficiency using siRNA against <i>ATF6, CHOP, JNK</i> and <i>XBP1</i> by qPCR. (B) miR-24 expression level was not altered upon knockdown of Caspase 3, Caspase 8, Caspase 9, Caspase 12, ATF6, CHOP, JNK or XBP1. (C) Knockdown of CHOP but not other factors in the ER stress pathway partially rescued increased apoptosis caused by miR-24 inhibition. *, p<0.05. Data were analyzed by unpaired Student's t test. (D) PCR for <i>Bim</i> showing decreased <i>Bim</i> expression upon CHOP knockdown. *, p<0.05. Data were analyzed by unpaired Student's t test. (E) Western for Bim showing decreased Bim protein level when CHOP siRNA was transfected into primary CMs. GAPDH serves as a loading control.