Hindlimb suspension and/or 2 Gy of proton radiation increases serum total corticosterone levels.

<p>Groups of 10 C3H/HeN female mice per treatment group were placed in hindlimb suspension and/or proton irradiated (2 Gy). Five days later, serum was obtained and analyzed for total corticosterone by ELISA. Data from 10 mice in each group were averaged and error bars are standard error of the mean. C = control, S = hindlimb suspended, R = irradiated, and R+S = irradiated and suspended. Statistical significance of the effect of hindlimb suspension and irradiation and the interaction between them was calculated by 2-way ANOVA.