Proton irradiated mice treated with hindlimb suspension fail to control a challenge with <i>Pseudomonas aeruginosa</i>.

<p>Groups of 10 C3H/HeN male (A) and female (B) and Balb/c male (C) and female (D) mice were proton irradiated (2 Gy) and/or placed in hindlimb suspension. Five days later, mice were exposed to <i>Pseudomonas aeruginosa</i> by intraperitoneal injection and followed for 5 days. Morbidity scores (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085665#pone-0085665-t001" target="_blank">Table 1</a>) were calculated daily and animals were considered morbid if their score increased by 3 points or remained elevated 2 points for 24 hrs. C = control, S = hindlimb suspended, R = irradiated, and R+S = irradiated and suspended. Statistical significance was measured by log rank analysis of Kaplan-Meier curves and expressed in the table as column versus rows.