Identification of ACE2 peptides by LC-MS/MS in the 60 kDa deglycosylated band from the media of mouse PT cells.

<p>(A) Mass spectrometry identified peptides matched with the mouse ACE2 sequence (SWISS-PROT database no. Q8R0I0) at positions 18-577. The identified peptides significantly matched with mouse ACE2 sequences are underlined and shown in bold. The peptides matched with mouse ACE2 sequence, but not statistically significant, are underlined only (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085958#pone.0085958.s006" target="_blank">Table S3</a> for detailed analyses of peptides). The overall sequence coverage is 24%. (B) Tandem mass spectrum of the <i>C</i>-terminal peptide (ALENVVGAR 569-577). The b and y ions result from the cleavage of peptide bonds and correspond to <i>N</i>-terminal and <i>C</i>-terminal fragments of the peptide, respectively. The detected b and y ions are consistent with the peptide sequence shown on the top part of the panel. Ions score: 59, Observed ion: 464.77, Mr(expt): 927.52, Mr(calc): 927.51. Expect value: 0.0002 (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085958#pone.0085958.s006" target="_blank">Table S3</a> for details).