Serum mapping against different HIV-1 epitopes.

<p>Sera of HIV-1 patients were tested for binding against different HIV-1 antigens including recombinant gp120_JR-FL, gp41_JR-FL, and p24 (A). To check for MPER specific antibodies, serum binding against three different MPER peptides was evaluated (B). To gain better knowledge about the binding epitopes on gp120, sera were tested against the gp120 variable loop truncation variants ΔV1/V2 and ΔV3 (C). Endpoint titers of the patient samples were determined by calculating the highest serum dilution that gives a reading above the blank including three standard deviations.