IFN-γ and IL-17 are not produced by CD4⁺CD25^hi iTreg cells in lepromatous leprosy.

<p>Antigen stimulated PBMC cultures of 3 each of lepromatous and tuberculoid patients were investigated by flowcytometry for the presence of IFN-γ and IL-17 in CD3⁺ gated CD4⁺CD25^hi cells. Representative data on each of tuberculoid and lepromatous leprosy patients shows <,1% of IFN-γ+ cells in both leprosy groups. BT showed low percentage of cells with IL-17 which was further decreased in LL. That the negligible cytokine detection was not due to trivial reasons was indicated by CD25-ve population which showed both IFN γ and IL 17, which were as expected higher in tuberculoid as compared to lepromatous subjects. Abbreviations as in legends to <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002639#pntd-0002639-g002" target="_blank">Figures 2</a> and <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002639#pntd-0002639-g003" target="_blank">3</a>.