hBM-derived SB cells differentiate <i>in vitro</i>.

<p><b>A) SB cell attachment.</b> Purified Lgr5+ cells at day 0 (left) and after attachment (right). <b>B) Mesoderm differentiation.</b> Before (left) and after (right) Oil Red O staining for differentiated adipocytes. <b>C) Quantification of Oil Red O- stained cells.</b> Negative controls: SB cells cultured in adipogenesis medium only and SB cells cultured in Stem Pro 34 medium, which is not an adipocyte differentiation media. <b>D) Endoderm differentiation.</b> RT-PCR assay for hepatocyte formation; “-” indicates the negative controls without template cDNA. Expected product sizes: GAPDH (2), 184 bp; albumin, 152 bp; FoxA2, 164 bp; and alpha-fetoprotein, 272 bp. <b>E) Ectoderm differentiation.</b> RT-PCR assay for differentiated neurons; “-” indicates negative controls without template cDNA. Expected product sizes: GAPDH (2), 184 bp; MAPT, 235 bp; and NEFL (neurofilament), 184 bp. Note: * indicates P≤0.01. <b>F) Immunocytochemistry using neurofilament (Cyc-3) staining.</b> Filters (left to right): red (Cyc-3), UV (DAPI), FITC (background), and merged Cyc-3 + DAPI. All scale bars, 100 µm.