Effects of different normalization approaches on the expression of miR-221 and miR-29b.

<p>Fold expression changes of miR-221 (dark grey columns) and miR-29b (light grey columns) in MSG-obese mice were normalized to individual or combined reference genes. Error bars show the standard error calculated from three biological replicates. Stars indicate the significant (P<0.05) difference identified by uncorrected Fisher’s LSD test in multiple comparisons after two-way ANOVA.