Exogenous bFGF inhibits thawing efficiency in a dosage dependent manner across all three-cell lines.

<p>Identical wells of H1-0 ng, H9-0 ng or DF19-0 ng culture were each frozen individually into a single vial after dissociation by collagenase or accutase. Subsequently each vial of frozen cells was plated equally into 0, 0.04, 0.4 or 4 ng/ml bFGF media, 3 wells of each. The average number of colonies from triplicate wells was presented for each culture (n = 2). Error bars represent standard deviation. Asterisks indicate statistical differences between different bFGF culture conditions in the accutased group. **: P<0.1; *: P<0.01. See also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0086031#pone.0086031.s002" target="_blank">Figure S2</a>.