<p>Specific lysis of autologous PHA blasts loaded with CMVpp65 peptides at E:T ratios from 20∶1 to 0.5∶1 was determined using fluorescent dye Calcein-AM cytotoxicity assay in CD25+ expanded cells. Graphs show pooled data from all cytotoxicity experiments in G-CSF-mobilized (A; <i>n</i> = 4) and non-mobilized (B; <i>n</i> = 4) CD25+ expanded CMV-T.</p
OBJECTIVE: To identify an immunodominant HLA-A33-restricted epitope within the CMV matrix phosphopro...
<p>PBMC from 4 healthy CMV pp65 responding donor were stimulated for 8 day with a mixture of 15 amin...
<p>(<b>A</b>) Schematic representation of the assay. Balb/c mice inoculated with pcTPANS1 or pcTPA p...
<p>Expanded CD25+ cells isolated from both G-CSF-mobilized and non-mobilized donors were stimulated ...
<p>0.25×10<sup>6</sup> CD25+ T cells were expanded in culture for up to 24 days in the presence of I...
<p>Cells were incubated with PHA and the two concentrations of Tofa (grey histograms); after four da...
Cytomegalovirus (CMV)-specific T cells expand with CMV reactivation and are probably prerequisite fo...
<p>(<b>A</b>) Clonotype frequencies from sorted proliferating CD8<sup>+</sup> T cells following CMV ...
<p>Expanded CMV pp65<sub>aa495–503</sub> specific T cells of 2 HD (•,▴) and a CMV pp65<sub>aa495–503...
<p>(a) Cytotoxicity effects were analyzed by MTT. The data represents as the mean ±SD, and acquired ...
<p>(A) The results depict representative data from a flow cytometry-based complement-mediated cytoto...
<p>(<b><i>a</i></b>) Flow cytometric analysis of 14-day expansion culture of purified Vδ1+ T cells f...
<p>A CFSE based cytotoxicity assay was set up as follows: target cells were the original priming APC...
<p>The cell viability was assessed by the MTT assay in Hep G2 cells treated with up to 128 µM Cd<sup...
<p>(A) The schematic illustrates the amino acid sequence of each CPPP derivative. (B–C) MDA-MB 468 c...
OBJECTIVE: To identify an immunodominant HLA-A33-restricted epitope within the CMV matrix phosphopro...
<p>PBMC from 4 healthy CMV pp65 responding donor were stimulated for 8 day with a mixture of 15 amin...
<p>(<b>A</b>) Schematic representation of the assay. Balb/c mice inoculated with pcTPANS1 or pcTPA p...
<p>Expanded CD25+ cells isolated from both G-CSF-mobilized and non-mobilized donors were stimulated ...
<p>0.25×10<sup>6</sup> CD25+ T cells were expanded in culture for up to 24 days in the presence of I...
<p>Cells were incubated with PHA and the two concentrations of Tofa (grey histograms); after four da...
Cytomegalovirus (CMV)-specific T cells expand with CMV reactivation and are probably prerequisite fo...
<p>(<b>A</b>) Clonotype frequencies from sorted proliferating CD8<sup>+</sup> T cells following CMV ...
<p>Expanded CMV pp65<sub>aa495–503</sub> specific T cells of 2 HD (•,▴) and a CMV pp65<sub>aa495–503...
<p>(a) Cytotoxicity effects were analyzed by MTT. The data represents as the mean ±SD, and acquired ...
<p>(A) The results depict representative data from a flow cytometry-based complement-mediated cytoto...
<p>(<b><i>a</i></b>) Flow cytometric analysis of 14-day expansion culture of purified Vδ1+ T cells f...
<p>A CFSE based cytotoxicity assay was set up as follows: target cells were the original priming APC...
<p>The cell viability was assessed by the MTT assay in Hep G2 cells treated with up to 128 µM Cd<sup...
<p>(A) The schematic illustrates the amino acid sequence of each CPPP derivative. (B–C) MDA-MB 468 c...
OBJECTIVE: To identify an immunodominant HLA-A33-restricted epitope within the CMV matrix phosphopro...
<p>PBMC from 4 healthy CMV pp65 responding donor were stimulated for 8 day with a mixture of 15 amin...
<p>(<b>A</b>) Schematic representation of the assay. Balb/c mice inoculated with pcTPANS1 or pcTPA p...