Effect of HSPB1 cleavage on tumor progression.

<p><b>A</b> B16F10 melanoma cell lines were stably transfected with PPTLS, sHSPB1WT, sHSPB1 (59–203), and HSPB1WT. Cell lysates and conditioned media of two stable lines (1,2) were repectively subjected to western blotting (top). <b>B</b> Effects of conditioned media of B16F10 stable cell lines on VEGF₁₆₅-induced VEGFR2 activation and proliferation activity in HUVECs. <b>C</b> Mean number of metastases at 14 days after intravenous injection of B6F10 stable cells into wild-type mice. The graph shows the mean ± SEM from three independent experiments of six mice per group f (*<i>P</i> <0.05 and **<i>P</i> <0.01 vs PPTLS). Right panel shows representative images. <b>D</b> Intratumoral microvessel density (MVD) per high-powered field (h.p.f.) was quantified by CD31 immunofluorescence. (*<i>P</i> <0.05 and **<i>P</i> <0.01 vs PPTLS). <b>E</b> Mean weight of liver with tumor at 14 days after intrasplenic injection of B6F10 stable cells into wild-type mice.