Effect of <i>SCO4676</i> and <i>SCO4677</i> deletion on the expression of scr4677.

<p>A) Northern blot analysis of scr4677 expression in wild type and <i>SCO4676</i> deletion (apramycin-containing) strains during development on rich R2YE medium. 5S rRNA was used as a control for RNA abundance and RNA integrity. B) Northern blot analysis of scr4677 as described in A), only wild type and mutant strains carried <i>scr4677</i> on a high copy number plasmid (pWHM3). C) Electrophoretic mobility shift assay of a [γ³²-P] radiolabelled 83 bp probe containing the <i>scr4677</i> upstream region (extending from +77 to +159 nt relative to the scr4677 transcription start site), following incubation with cell free extracts from the strains indicated. Binding specificity was tested using excess amounts of specific (non-radiolabeled probe) and non-specific competitor (sequence within <i>SCO3287</i>) DNA. D) Northern blot analysis of scr4677 expression during growth on rich (R2YE) medium in a wild type and <i>SCO4677</i> deletion mutant strain. As above, 5S rRNA was used as a loading control.