Organization, conservation and expression of scr4677.

<p>A) Schematic illustration (not to scale) of the genetic region surrounding <i>scr4677. scr4677</i> is located 14 nt upstream of <i>SCO4676</i>, in the 248 nt intergenic region between <i>SCO4677</i> and <i>SCO4676</i>, encoded on the strand opposite these two protein-coding genes. B) and C) <i>scr4677</i> is found upstream of <i>SCO4676</i> orthologues in many <i>Streptomyces</i> species, including <i>S. coelicolor</i> (left, where <i>SCO4676</i> is co-transcribed with both <i>SCO4677</i> and <i>SCO4675</i>), <i>S. avermitilis</i> (middle) and <i>S. venezuelae</i> (right). In B), the green colour denotes gene expression from the negative (antisense) strand, while red indicates expression from the positive (sense) stand. In C) expression of scr4677 (left) and its orthologues sar3140 (middle) and svr3556 (right) was investigated using RNA harvested throughout the developmental cycle (first timepoint: vegetative growth; second timepoint: aerial hyphae/fragmentation; third timepoint: sporulation) using northern blot analysis. D) Northern blot analysis of scr4677 expression in <i>S. coelicolor</i> following growth on minimal medium (MM) or rich (R2YE) medium for the times indicated. E) Northern blot analysis of scr4677 expression in <i>S. coelicolor</i> following growth on soy flour medium supplemented either with mannitol (SFM; left) or glucose (SFG; right), for the times indicated. For each northern blot, 5S rRNA served as the control for RNA loading and RNA integrity.