Adipokine secretion in the trans-well versus contact co-culture system.

<p>Adipokine secretion after the 12-culture incubation in the contact (A) and trans-well (B) treated co-culture conditions, and (C) a comparison of secreted MCP1, IL-6 and TNFα between the contact and trans-well co-culture systems. Note: no IL-10 was detected in the trans-well system (B). 0 hr = serum starved adipocytes alone prior to co-culture and fatty acid treatment, (−) = negative control; adipocytes alone treated with 25 µM BSA, (+) = positive control; co-cultured adipocytes and macrophages plus 25 µM BSA, DHA = co-cultured adipocytes and macrophages in the presence of 125 µM DHA, EPA = co-cultured adipocytes and macrophages in the presence of 125 µM EPA, and PA = co-cultured adipocytes and macrophages in the presence of 125 µM PA. Values are means ± SEM. The experiment was independently conducted 3 times for a final sample size of n = 6–9. A different letter or an asterisk (*) indicates treatments are significantly different from each other, p≤0.05.