M1 and M2 macrophage polarization marker mRNA expression in trans-well co-cultured macrophages.

<p>mRNA expression of key (A) M1 (<i>Nos2</i>, <i>Nfκb</i> and <i>Tnfα</i>), (B) M2 (<i>Arg1</i>, <i>Mrc2</i>) polarization genes, and (C) regulatory cytokines (<i>Tgfβ1</i> and <i>Il-10</i>) from macrophages harvested from the trans-well system at 12 hr. 0 hr = serum starved adipocytes alone prior to co-culture and fatty acid treatment, (+) = positive control; co-cultured adipocytes and macrophages plus 25 µM BSA, DHA = co-cultured adipocytes and macrophages in the presence of 125 µM DHA, EPA = co-cultured adipocytes and macrophages in the presence of 125 µM EPA, and PA = co-cultured adipocytes and macrophages in the presence of 125 µM PA. Values are mean fold change ± SEM. The experiment was independently conducted 2 times (in triplicate) for a final sample size of n = 6. A different letter indicates treatments are significantly different from each other, p≤0.05.