Primer Sequences and Cycling Conditions.

a<p>Construct name. The numbers in parentheses indicate the NS4B amino acid (aa) from 1–255 and the minus sign represent the number of aa to C-teminal ends of NS4A preceding NS4B protein.</p>b<p>Primer name. The CT or NT in the primer name denotes C- or N-terminal end of NS4B with respect to GFP fusion protein whereas the F or R denotes the sense or antisense primer, respectively.</p>c<p>The Kozak sequence is in bold, lowercase and italicize. The nucleotides in lower case only are codon filling nucleotides. The viral sequences are in uppercase.</p>*<p>GenBank Accession No. AF196835.