Synergism between phenformin and oxamate in mediating cancer cell death.

<p>(A) E6E7Ras cells were treated for 2 days with oxamate at the indicated concentrations (0–80 mM) and then dead cells were counted by flow cytometry. (B, C) The indicated cells lines were treated with varying concentrations of phenformin, oxamate, or combinations of the two drugs. In (B) cells were treated for 1, 2, or 3 days prior to counting dead cells. In (C) cells were treated for 24 hours before determining number of dead cells. C: control, P: phenformin, O: oxamate, PO: phenformin+oxamate. In (C) the numbers below each bar indicate concentrations of each drug in mM (e.g., P0.5O20 means P 0.5 mM+O 20 mM). * indicates a synergistic effect in the group PO compared with the other groups.