Serum leptin in primiparous and pluriparous as well as leptin mRNA data (LEP) in pluriparous cows.

<p>Leptin serum concentrations (upper graph) and leptin mRNA abundance in s.c. tail head adipose tissue (lower graph) in pluriparous and primiparous cows receiving conjugated linoleic acids (CLA, Lutrell® Pure, BASF SE, Ludwigshafen, Germany) at 100 g/day (CLA) or a control fat supplement (Silafat®, BASF SE) from day 1 until day 182 postpartum in Trial 1. All animals and samples were included for serum data [Control: pluriparous cows n = 10, primiparous cows n = 5; CLA: pluriparous cows n = 11, primiparous cows n = 5]. For mRNA data only pluriparous cow samples from days −21, 1, 21, 70, 105 182 196, 224, and 252 relative to parturition for control group and days −21, 21, 105, 196, and 252 for CLA group were analysed. For normalisation, lipoprotein receptor-related protein 10 (LRP10), RNA Polymerase II (POLR2A) and glyceraldehyde-phosphate-dehydrogenase (GAPDH) in s.c. adipose tissue were used as reference genes. ns: not significant. Different letters indicate significant differences between days relative to parturition (<i>P</i>≤0.05; mean ± SEM). Area between vertical lines corresponds to the CLA supplementation period. A.U.: arbitrary units.