Rev1 forms a complex with Pol31 and Pol32.

<p>(A) Purity of the proteins. We analyzed 0.5 µg of each protein on a 10% denaturing SDS-polyacrylamide gel. Molecular mass standards are shown on the right. (B) GST pull-down of the purified proteins. GST–Pol32 immobilized on glutathione–Sepharose beads was incubated with purified Pol31 and Rev1. After washing, bound proteins were eluted with glutathione. Aliquots of each sample, taken before addition to the beads (L), from the flow-through fraction (F), from the last wash (W), and from the glutathione-eluted proteins (E), were analyzed on 10% SDS-polyacrylamide gel (lanes 1–4). The results for the control experiment using GST instead of GST–Pol32 are shown in lanes 5–8. Molecular mass standards are shown on the right.