Ly6C^hi monocytes mature <i>in situ</i> into Ly6C^low wound macrophages.

<p>Sponges were implanted into CD45.1 congenic mice. At 1 day post-wounding, sponges were transferred from donor CD45.1 congenic mice to recipient B6 (CD45.2) mice as described in Materials & Methods. Wound cells were isolated from recipient mice at 1, 3 or 7 days post-transfer and monocyte/macrophage subsets were analyzed by flow cytometry. CD45.1⁺ cells were identified on gated F4/80⁺SSC^low/int cells and examined for F4/80⁺Ly6C^hi and Ly6C^low subsets. Numbers accompanying gates are the mean frequency ± SD, n = 3 mice per group. Data are representative of at least 2 independent experiments.