Analytical gel chromatography of zfNAGS-C with and without L-arginine.

<p>The top panel shows a semi-logarithmic plot of molecular mass vs. elution volume. Open circles correspond to elution volumes of blue dextran (2000 kDa), ferritin (440 kDa), catalase (232 kDa), aldolase (158 kDa), bovine serum albumin (66 kDa), ovalbumin (43 kDa), and myoglobin (16 kDa). Lower panels show absorption at 280 nm as a function of elution volume. The concentration of zfNAGS-C loaded on the column is indicated in each panel. Dark blue - elution profiles of zfNAGS-C without L-arginine. Cyan – elution profiles of zfNAGS-C in the presence of 1 mM L-arginine.