Increased DNA methyltransferase gene expression in prostate cancer cell lines was decreased with SFN or DIM treatments.

<p>(A) Gene expression of DNMT1, DNMT3A, and DNMT3B in untreated PrEC, LnCAP, and PC3 cells (n = 3–5 per group). Data represent mean normalized fold-change ± SEM compared to PrEC. (B–D) Effects of SFN and DIM on DNMT gene expression in PrEC cells (B), LnCAP cells (C), and PC3 cells (D). Cells were treated with vehicle control (DMSO), 15 µM SFN, or 15 µM DIM (n = 6 per group in B–D). DNMT1, DNMT3A, and DNMT3B gene expression were analyzed 48 h post-treatment. Data represent mean normalized fold-change ± SEM compared to DMSO. *p-value <0.05.