URT cells express ALDH1A mRNA.

<p>RNA was extracted from cells and cDNA was synthesized using oligo-dT₂₀ primers. Serial 1∶10 dilutions of the cDNA were used for PCR amplifications. Gels were loaded from left to right with PCR products from serially diluted cDNA. The left-most columns were representative of products from ∼1×10³ cells. <b>Panel A.</b> Results are shown for NT cells (see Materials and Methods), cervical lymph nodes (CLN) and mesenteric lymph nodes (MesLN) of naïve C57BL/6 mice, separated into CD11c^Hi and CD11c^Lo/neg populations and tested for ALDH1A2 and GAPDH mRNA. <b>Panel B.</b> CD11c^Hi and CD11c^Lo/neg NT populations were tested for ALDH1A1, ALDH1A2, ALDH1A3 and GAPDH mRNA. <b>Panel C.</b> Cells were tested for ALDH1A2 mRNA. Samples included NT cells that had been FACS-sorted for the F4/80⁺CD11c^-CD11b⁺ phenotype (abbreviated ‘F4/80+CD11c-’), two macrophage lines MAC INF4.29 and LIE 13–14, NT cells or lung cells enriched for epithelium by negative selection and short-term culture (see Materials and Methods), and LET cells. On a per-cell basis, the highest ALDH1A expression levels were among CD11c^Lo/neg cell populations.