The DA-actin exodus is not blocked by inhibitors of myosin light chain kinase (MLCK) or Rho-associated kinase (ROCK).

<p>Neurons (21 DIV) were preincubated with 10 µM of ML-7, an inhibitor of MLCK (A) or 1 µM H-1152, an inhibitor of ROCK (B) for 30 min, and then stimulated with 100 µM glutamate for 10 min. Neither ML-7 (n = 30 cells; drebrin SDR p<0.01, actin SDR p<0.01, Scheffe's test) nor H1152 (n = 30 cells; drebrin SDR p<0.01, actin SDR p<0.01, Scheffe's test) blocked the DA-actin exodus. F-actin images indicate that spines kept their structure during the experiment although their shapes were changed. Scale bars, 5 µm.