Members of diaphanous related formin (DRF) family are required to mediate regulation of epithelial plasticity by p115RhoGEF.

<p>A) p115-OE MCF7 cells were transfected with SMARTpool™ siRNA or individual siRNA oligos targeting <i>DIAPH1</i>. <i>DIAPH1</i>-depleted p115-OE MCF7 cells showed decreased junctional E-cadherin as compared to <i>DIAPH1-</i>expressing p115-OE MCF7 cells. B) p115-OE MDA-MB-231 cells were treated with SMARTpool™ siRNA or individual siRNA oligos targeting <i>DIAPH1</i>. <i>DIAPH1</i> knockdown blocked the change in morphology seen in cells that overexpressed p115RhoGEF. C) Quantitation of E-cadherin ratio in p115-OE MCF7 cells showed a significant decrease in upon <i>DIAPH1</i> knockdown as compared to cells that were treated with control siRNA (mean ± SE). 90 cell-cell junctions were analyzed from 3 experiments. D) Quantitation of morphology in terms of circularity in p115-OE MDA-MB-231 cells showed a significant change upon <i>DIAPH1</i> knockdown as compared to cells that were treated with control siRNA (mean ± SE). 75 cells were analyzed from 3 experiments E) Representative immunoblots showing knockdown of <i>DIAPH1</i> upon treatment with <i>DIAPH1</i> oligo#1, <i>DIAPH1</i> oligo#2 and <i>DIAPH1</i> SMARTpool™ siRNA in MCF7 or MDA-MB-231 cells that overexpressed p115RhoGEF. Scale bars represent 10 µm.