Carbon source utilization by different <i>L. mexicana</i> developmental stages.

<p>L. mexicana Pro^log, Pro^stat and Ama^axenic were suspended in chemically defined medium (CDM) containing ¹³C-U-glucose (A) or ¹³C-glutamate (B) and the rate of utilization of the proffered ¹³C-carbon source (left hand panel) and production of overflow metabolites (right hand panel) assessed by ¹³C-NMR analysis of the conditioned medium. ¹³CO₂ (detected as H¹³CO₃⁻) was only quantified in the medium of promastigote stages, as it is not retained in the acidified amastigote medium. C. Proportion of internalized ¹³C-glucose that was secreted as partially oxidized end-products by Pro^log, Pro^stat and Ama^axenic. Similar relative levels of secretion were observed in the two promastigote stages (∼50%), while negligible secretion was observed in Ama^axenic. D. Replication rates of different L. mexicana developmental stages. Pro^log, Pro^stat and Ama^axenic were cultivated in RPMI supplemented with D₂O (5% v/v, final) and the rate of DNA turnover determined by kinetic analysis of deuterium incorporation into adenosine deoxyribose. All data represent mean (n = 3) and standard deviation and are representative of several independent experiments.