Fatty acid β-oxidation is increased in <i>L. mexicana</i> Ama^axenic.

<p>A. <i>L. mexicana</i> promastigote and amastigote stages were suspended in CDM supplemented with ¹³C-U-fatty acids for 3 h and isotopic enrichment (mol percent) in intermediates of central carbon metabolism was determined by GC-MS. Abbreviations as for <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003888#ppat-1003888-g002" target="_blank">Figure 2</a>. This data (mean (n = 3) and SD) is also presented in Tables S7 in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003888#ppat.1003888.s003" target="_blank">Text S1</a>. B. Mass isotopomers of citrate and glutamate in ¹³C-U-fatty acid fed (3 h) <i>L. mexicana</i> Ama^axenic. C. Wild type (WT) Pro^log and Ama^axenic and glycocalyx deficient Δ<i>pmm</i>-Pro^log parasites were labeled with ¹³C-U-fatty acids for 48 h and isotopic enrichment in the total lipid fraction (exemplified by hexadecanoic acid, C18:0) and citrate was determined by GC-MS. D. Fatty acid β-oxidation is elevated during glucose starvation. <i>L. mexicana</i> Pro^log, Pro^stat and Ama^axenic were labeled with ¹³C-U-fatty acids for 3 h in glucose-replete (+Glc) or glucose-free (−Glc) CDM and isotopic enrichment in intracellular pools of citrate and glutamate determined by GC-MS. Data represent mean (n = 3) and SD.