Isotopic enrichment of intracellular metabolite pools following cultivation with different ¹³C-labeled carbon sources.

<p>A. <i>L. mexicana</i> Pro^log, Pro^stat and Ama^axenic were suspended in CDM containing either ¹³C-U-glucose (Glc), a mixture of ¹³C-U-amino acids (AA), ¹³C-U-alanine (Ala), ¹³C-U-aspartate (Asp), or ¹³C-U-glutamate (Glu) for 3 h and ¹³C-enrichment (mol percent) in intracellular intermediates in central carbon metabolism determined by GC-MS. Data shown for ¹³C-Glc and individual amino acid labeling in Pro^log panel was taken from <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003888#ppat.1003888-Saunders1" target="_blank">[18]</a>. Mean (n = 3) and SD for this data is provided in Tables S1–6 in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003888#ppat.1003888.s003" target="_blank">Text S1</a>. Abbreviations used are as follows G6P, glucose 6-phosphate; F6P, fructose 6-phosphate; S7P, seduheptulose 7-phosphate; Ru5P, ribulose 5-phosphate; 3PG, 3-phosphoglycerate; 2PG, 2-phosphoglycerate; PEP, phosphoenolpyruvate; Suc, succinate; Mal, malate; Fum, fumarate; Cit, citrate; Ala, alanine; Asp, aspartate; Glu, glutamate; Gly, glycine; Ser, serine; Thr, threonine; Pro, proline; Ile, isoleucine; Leu, leucine; Lys, lysine; Phe, phenylalanine; Val, valine; Put, putrescine; Orn, ornithine; MTA, 5-methylthioadenosine; Ura, uracil; CHO1, mannogen; I3P, inositol 3-phosphate; MI, <i>myo</i>-inositol; G3P, glycerol 3-phosphate.