Time courses of single transport studies of piroxicam across PBMEC/C1-2, ECV304 and RBMEC layers.

<p>Comparison between the cleared volume vs. time graphs of internal standards for the transcellular transport route (diazepam, 100 µM), the paracellular transport route (carboxyfluorescein, 5 µM) and the NSAID piroxicam (100 µM) showed clearly that piroxicam permeated between the two markers across all three BBB (A: PBMEC/C1-2; B: ECV304; C: RBMEC) in vitro models. In addition, it was proved that as tighter the model is a wider frame between the two markers diazepam and carboxyfluorescein could be provided to analyze and compare permeabilities of single drugs. (n = 3 for each time point, data are presented as means ± SD).