MAP1S is required for flagellin to inhibit breast cancer cell proliferation.

<p>(A) The cell proliferation of MCF-7/shCtrl and MCF-7/shMAP1S cells was determined using the MTT assay after treatment with 0.5 µg/ml flagellin for the indicated number of days. (B) Four representative 35-mm plates of MCF-7/shCtrl and MCF-7/shMAP1S-2 cells were cultured for 8 days with or without flagellin treatment. Cells were then stained with 1 mg/ml MTT solution for 30 min and images of the colonies that formed were captured with a microscope. The number of colonies (≥100 µm in diameter) was counted using Image-Pro Plus software. **indicates <i>p</i><0.001, relative to untreated cells. (C) MCF-7/shCtrl and MCF-7/shMAP1S-2 cells were stimulated with 0.5 µg/ml flagellin for the indicated number of days. DNA content was assessed by PI staining and flow cytometry. Quantitative data showing different phases of the cell cycle is displayed. (D) MCF-7/shCtrl and MCF-7/shMAP1S-2 cells were treated with 0.5 µg/ml flagellin for the indicated time periods, and Cyclin D1 and p27 level were analyzed by western blotting with specific antibodies. All data are representative results of 3 independent experiments.