Acute HFD activates c-Fos in specific nucleus of mesolimbic pathway.

<p>Panel <b>A</b> shows a schematic diagram of the brain regions under study in a coronal section of the mouse brain (left column) and representative microphotographs of c-Fos immuno-staining of control, HFD-<i>ad lib</i> and HFD-pair-fed groups. Upper, middle and bottom line of images show the NAc (core and shell), the CeA and the LHA, respectively. Scale bar: 100 µm. Panels <b>B-E</b> show quantitative analysis of c-Fos staining in the NAc core (B), NAc shell (C), CeA (D) and LHA (E). Histograms depict the total number of c-Fos-IR neurons, expressed as cells per coronal section, for each experimental group. Values are the mean±SEM. **, p<0.01 vs. control group. ***, p<0.001 vs. control group.