Expression analysis of <i>EDNRB2</i> by quantitative real-time RT-PCR in three-day-old chicks with the wild-type (<i>Mo</i>⁺), white (<i>mo^w</i>) and mottled (<i>mo</i>) plumage.

<p>(A) Relative expression levels in skin tissues of chicks with the wild-type (<i>Mo</i>⁺/<i>Mo</i>⁺) (n = 9) (a) and white mutant (<i>mo^w</i>/<i>mo^w</i>) plumage (n = 6) (b, c) in the F₂ progeny that were obtained from the mating of a white Shokoku male (<i>mo^w</i>/<i>mo^w</i>) with a GSP female (<i>Mo</i>⁺/<i>Mo</i>⁺); and chicks with the wild-type (<i>Mo</i>⁺/<i>Mo</i>⁺) (n = 3) (d) and mottled type (<i>mo</i>/<i>mo</i>) plumage (n = 2) (e, f) in EJ line. Dotted circles indicate the regions from which skins were collected for analysis. Each bar shows the average expression level, which is presented as the quantity of gene expression relative to that of the wild type. (B) Expression level in liver tissues of the same individuals which were used for expression analysis of skins: the wild-type (<i>Mo</i>⁺/<i>Mo</i>⁺) (n = 9) and white mutant (<i>mo^w</i>/<i>mo^w</i>) chicks (n = 6) in the F₂ progeny between GSP and white Shokoku; and the wild-type (<i>Mo</i>⁺/<i>Mo</i>⁺) (n = 3) and mottled (<i>mo</i>/<i>mo</i>) plumage (n = 2) in EJ. Asterisks indicate a statistically significant difference at <i>P</i><0.05 (one-way ANOVA followed by Tukey HSD test). The vertical bars indicate standard deviations.