MnSOD in 3T3-L1 cells differentiated in the presence of free fatty acids.

<p>(A) Photomicrograph of 3T3-L1 cells differentiated with the standard protocol and in the presence of 200 µM palmitate (PA), oleate (OA) or linoleate (LA). (B) MnSOD in 3T3-L1 cells differentiated with the standard protocol (C) and in the presence of 200 µM PA, OA or LA. MnSOD in 3T3-L1 cells differentiated in the presence of increasing concentrations of PA (C), OA (D) or LA (E). (F) Quantification of the data of 3 PA incubated, 4 OA incubated and 3 LA incubated cells partly shown in B to E. (G) MnSOD, FABP4 and Cox-4 protein in preadipocytes (Pre) and cells differentiated for 1 d, 2 d, 3 d, 6 d and 9 d in the presence (+) or absence (−) of 400 µM oleic acid. (H) MnSOD, FABP4, Cox-4 and GAPDH in 3T3-L1 cells differentiated in the presence of 200 µM PA, OA or LA. (I) Full length (PARP, 116 kDa) and cleaved PARP (PARP, 89 kDa) in 3T3-L1 cells differentiated in the presence of 200 µM PA, OA or LA. ST indicates lysate of staurosporine treated 3T3-L1 cells as positive control which was analyzed on the identical gel in a non-adjacent lane. (J) Number of mature adipocytes when cells were differentiated in the presence of 200 µM PA, OA or LA. Data of 4 experiments are shown.