NXS/T multiplicity correlates with the evolutionary rates of secretory proteins.

<p>(A) Human-mouse and human-pufferfish amino acid identities for gene orthologues grouped by human NXS/T number, linear regression slopes are indicated, * Pearson correlation p<0.05. (B) Apparent evolutionary rates (<i>d</i>_N/<i>d</i>_S) of human-mouse orthologues grouped by site number. (C) Human SNPs in cytosolic and secretory proteins represented as <i>N/S</i>. (D) Slopes from NXS/T multiplicity versus amino acid identity (secreted-cytosolic)/separation time from human), were graphed for 52 animal species (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0086088#pone.0086088.s007" target="_blank">Fig. S7</a>)]. Pearson R² = 0.40; Spearman correlation R = 0.696. (E) Amino acid substitution rates are proportional to NXS/T site number. The slopes from human-mouse and human-pufferfish % identity were used to calculate substitutions.