Thymocyte selection in Egr3 TG mice favors γδ T cells.

<p>A-B) Thymocytes harvested from littermate Egr3 WT and Egr3 TG mice were stained for surface marker expression or lysed for mRNA quantification. A) CD4 vs. CD8 expression was measured to determine double negative, double positive, and single positive subsets (left panels). DN1, DN2, DN3, and DN4 subsets shown as a percentage of the double negative fraction (right panels). B) γδT cells shown as a percentage of thymocytes (left panels) and C) γδT cells shown as an absolute number. D) Expression of Id3 mRNA in DN1, DN2, DN3, DN4 thymocytes assessed by qRT-PCR and normalized to 18s expression. Data in A-D are representative of 3 independent experiments, each using 2 mice per genotype. **p ≤ 0.01, ***p ≤ 0.005