Level of HSV-1 immediate early, early, and CD80 in HSV-CD80 infected DCs.

<p>Subconfluent monolayers of DCs from C57BL/6 mice were infected with 1 PFU/cell of HSV-CD80 or parental virus as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0087617#pone-0087617-g001" target="_blank">Fig. 1</a>. For ICP-0 and TK measurements, cells were isolated 4, 8, and 12 hr PI, while for CD80 measurements cells were harvested 2, 4, 8, 12, 24, and 48 hr PI. Total RNA was isolated and TaqMan RT-PCR was performed using ICP0-, TK-, and CD80-specific primers as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0087617#s4" target="_blank">Materials and Methods</a>. ICP0 and TK mRNA levels were normalized in comparison to each transcript at 0 hr PI, while CD80 mRNA level was normalized to the level of CD80 in mock infected DCs. GAPDH was used as internal control. Each point represents the mean ± SEM (n = 6) from two separate experiments. Panels: A) ICP-0; B) TK, and C) CD80.