Coculture of HSV-CD80 infected DCs with naive WT T cells reduces CD3⁺PD-1⁺ expression.

<p>DCs from WT C57BL/6 mice were infected with 1 PFU/cell of HSV-CD80, parental virus, or mock-infected. At 24 hr PI, the infected DCs were incubated with T cells isolated from naive WT C57BL/6 mice at a 1:1 ratio. As a control some T cells were incubated without DCs. FACS analyses were carried out 24 post incubation using anti-CD3 and anti-PD-1 antibodies. Graphs show the contour of CD3⁺PD-1⁺ staining for each group. Panels represent HSV-CD80, Parental virus, Mock, and no DCs T cells. Number indicates the percent of PD-1⁺CD3⁺ expressing T cells per treatment. Experiments were repeated twice.