Comparison of the striped Aldoc or Venus expression pattern among wild type (<i>Aldoc</i>^+/+), heterozygote (<i>Aldoc</i>^+/Venus) and homozygote (<i>Aldoc</i>^Venus/Venus) mice.

<p>A–F, Photomicrographs of Aldoc immunostaining in a wild type (the most left column), Aldoc immunostaining, double labeling and Venus in a heterozygote (the three center columns), and Venus in a homozygote (the most right column) in horizontal sections of the cerebella at the similar levels (A, rostral cerebellum with three narrow zones; B, Paravermal lobule VIa; C, Lobule VIII; D, Junction between lobules IXc and X; E, Flocculus, dorsal part; F, Flocculus, ventral part). Arrowheads in D indicate areas with higher Aldoc/Venus expression in lobule X. White and black arrowheads in E and F indicate rostral and caudal parts of the flocculus that had different Aldoc/Venus expression levels. Sections from the wild type and heterozygote were processed with Aldoc immunostaining with Texas red-conjugated secondary antibody in the same session with the same antibody solutions. Photos of Aldoc immunostaining and Venus were, respectively, processed with the same exposure and adjustment settings for all mice in A–F. Scale bar under F applies to A–F. G, Higher magnification photomicrographs of Aldoc immunostaining (left panel), double labeling (center panel) and Venus (right panel) of a coronal section of the cerebellum (lobule VIII) in a heterozygote, showing complete match at the cell population level.