Anti-serotype A, B, and E ELISA titers in mice immunized by a trivalent protocol.

<p><b>A.</b> Immunization scheme <b>-</b> Ten female Balb/c mice were immunized s.c. with a trivalent vaccine containing 5 µg of each of the three Hc fragments, HcA, HcB, and HcE, in CFA (first injection) or IFA (second and third injections). Booster immunizations of trivalent or monovalent (HcE alone) vaccines were given i.m. at 4-week intervals. After three boosters, the immunization regimen was split into two (I and II). Then, two groups of five mice were differentially injected according to their ELISA titers. Mice were bled for titer analysis ten days after each immunization. <b>B</b>. ELISA titer development from week 13 through week 27 for the two combined protocols. As no significant difference in ELISA titer was observed between the two immunization groups (I and II), the data represent the geometric mean of all 10 mice with 95% confidence levels. <b>C</b>. Final titers of anti-serotype A, B, and E (at week 27) in the trivalent combined protocols compared to titers of anti-serotype A in the HcA monovalent control. Geometric means with 95% confidence levels are presented for each of the 10 animal groups (trivalent protocol: anti-A –23,600, anti-B –32,000, anti-E –28,500; monovalent protocol: anti-A –25,900).