Manual RT-PCR measurements of 3 selected miRNAs agree very well with measurements performed by TLDA assay.

<p>The control and schizophrenia samples of Experiment 1 were measured using individual TaqMan primer sets for miR-17, miR-145-5p and miR-219-2-3p. Note that lower Ct values indicate higher abundance on a log2 scale (i.e., a decrease of 0.5 Ct represents a 41% increase in abundance or 1.41-fold difference across groups). Error bars indicate standard error of the mean. In this assay, miR-17-5p showed a 1.18-fold increase (p = 0.05), which is very close to 1.22-fold reported in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0086469#pone-0086469-t001" target="_blank">Table 1</a>. miR-145-5p showed a 0.85-fold change (p = 0.16), similar to 0.68-fold in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0086469#pone-0086469-t001" target="_blank">Table 1</a>. miR-219-2-3p showed a 1.60-fold increase (p = 0.029), similar to 1.48-fold in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0086469#pone-0086469-t001" target="_blank">Table 1</a>.