Drought-responsive genes in soybean exhibit diurnal regulation, and the expression pattern is modified under drought stress.

<p>Gene expression data regards qPCR analysis of soybean leaves during moderate (A) and severe (B) drought. Expression axis represents normalized expression (NE) = 2∧^{-(Ct experimental – Ctn)}. Collect time points are represented by ZT (Zeitgeiber Time) 0 to 20, starting from the time the lights came on ( ZT0) and proceeding with 4 h intervals until ZT20. For easy viewing, asterisks represent significant differences between control and stressed plants in each time point (Duncan’ test 5%, <i>time -treatment</i> interaction). The ANOVA and the complete Duncan’s tests results can be found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0086402#pone.0086402.s006" target="_blank">Table S3</a>.