Effect of AEGT on the production and gene expression of proinflammatory cytokines in LPS-stimulated RAW 264.7 cells (A–F) and primary rat peritoneal macrophages (G–L).

<p>Cells were seeded in a 24-well plate treated with 400 or 800 µg/ml AEGT for 1 h, and then incubated with 1 µg/ml LPS for 24 h. The RNA (A–C and G–I and secreted protein levels of proinflammatory cytokines including TNF-α (D and J), IL1-β (E and K) and IL6 (F and L), were analyzed by qRT-PCR and ELISA, respectively. The efficiency of inhibition was determined as the percent RNA levels relative to those in the cells treated with LPS alone. Error bars indicate the means ± SD of three independent experiments. *<i>P</i><0.05; **<i>P</i><0.01.