2A1 and 2A1-Y179H variants characterization.

<p>(A) Temperature dependence of purified wild type PpAzoR (circles), 2A1 (diamonds) and 2A1-Y179H (triangles) variants. Reactions were performed in 100 mM sodium phosphate buffer, pH 7, in the presence of 100 µM AQS and 250 µM NADPH. (B) Light scattering of wild type PpAzoR (circles), 1B6 (squares) and 2A1-Y179H (triangles) variants. (C) Thermal inactivation of wild type PpAzoR (circles) and 2A1-Y179H (triangles) variant; enzyme samples were incubated at 50°C and activity measured at 30°C in aliquots taken at different time intervals.