Knockdown of TRP2 by shRNA does not affect p53 expression level.
- Roland Houben (96277)
- Corinna P. Schmid (348438)
- Melissa Maier (515060)
- Marion Wobser (515061)
- Stephanie Motschenbacher (515062)
- Jürgen C. Becker (259803)
- Claudia S. Vetter-Kauczok (515063)
- Gerhard Weyandt (515064)
- Sonja Hesbacher (348437)
- Sebastian Haferkamp (348443)
Publication date
January 2014
DOIAbstract
<p>(A) TRP2 and p53 expression in indicated melanoma cell lines was determined by western blot. Tubulin was used as a loading control. (B) The indicated melanoma cell lines were transduced with three different lentiviral TRP2 shRNAs (TRP2_#1, #2, #3) and on day four after shRNA infection the efficiency of the knockdown and p53 expression were analyzed by immunoblotting; a scrambled (scr) shRNA was used as a control.</p
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