Effects of TRP2 inhibition by shRNA on transcriptional activity of p53.

<p>Indicated melanoma cell lines were stably transduced with a lentiviral pGreenFire reporter construct encoding for green fluorescence protein (GFP) under the control of a p53 responsive element (4× CGACATGCCCGGGCATGT). The cells were then infected with the different lentiviral supernatants carrying the shRNA expression construct KH1 containing either a scrambled or a sequence targeting TRP2. Mean GFP activity four days post infection is depicted, normalized to the relative vector load of the cell lines determined by Real time PCR.