PFGE and Southern blot hybridization of undigested bacterial DNA from the different C. botulinum strains and outbreak isolates to identify boNT gene location.

C.botulinum strains and sequences used in this study.

Piyada Wangroongsarb (515097)
Tomoko Kohda (515098)
Chutima Jittaprasartsin (515099)
Karun Suthivarakom (515100)
Thanitchi Kamthalang (515101)
Kaoru Umeda (515102)
Pathom Sawanpanyalert (147633)
Shunji Kozaki (515103)
Kazuyoshi Ikuta (114419)

January 2014

aThe subtypes and GenBank accession numbers for strains determined in this study are indicated in...

Comparison of DNA fingerprinting methods for use in investigation of type E botulism outbreaks in the Canadian arctic

Daniel Leclair
Franco Pagotto
Jeffrey M. Farber
Brigitte Cadieux
John W. Austin

January 2006

Pulsed-field gel electrophoresis (PFGE), randomly amplified polymorphic DNA (RAPD) analysis, and aut...

PFGE and hybridization analysis.

Marie Kempf (184731)
Jean-Marc Rolain (34775)
Georges Diatta (241918)
Saïd Azza (40837)
Bissoum Samb (318668)
Oleg Mediannikov (199078)
Amy Gassama Sow (318669)
Seydina M. Diene (318670)
Florence Fenollar (74934)
Didier Raoult (3833)

February 2013

A. Pulsed field gel electrophoresis (PFGE) profiles obtained for the two Acinetobacter ...

Confirmation of tagging C. botulinum BoNT-encoding plasmids pBotCDC-A3-Erm (strain CDC-A3), pCLJ-Erm (strain 657Ba) and pCLL-Erm (strain Eklund 17B) by PFGE and Southern hybridization analysis.

Kristin M. Marshall (364207)
Marite Bradshaw (364208)
Eric A. Johnson (225183)

February 2013

(B) Ethidium bromide stained PFGE of nondigested DNA samples from C. botulinum stra...

Confirmation of plasmid pBotCDC-A3-Erm transfer from C. botulinum strain CDC-A3580s1 to strain LNT01 by PFGE and Southern hybridization analysis.

Kristin M. Marshall (364207)
Marite Bradshaw (364208)
Eric A. Johnson (225183)

February 2013

(A) Ethidium bromide stained PFGE of C. botulinum DNA samples: SmaI digested...

Confirmation of plasmid pCLJ-Erm transfer from C. botulinum strain 657BaCT4 to strain LNT01.

Kristin M. Marshall (364207)
Marite Bradshaw (364208)
Eric A. Johnson (225183)

February 2013

(A) Ethidium bromide stained PFGE of C. botulinum strains: LNT01 (Lanes 1 and 7), w...

Confirmation of plasmid pCLL-Erm transfer from C. botulinum strain Eklund 17BCT11 to strain LNT01.

Kristin M. Marshall (364207)
Marite Bradshaw (364208)
Eric A. Johnson (225183)

February 2013

(A) Ethidium bromide stained PFGE of C. botulinum strains: LNT01 (Lanes 1 and 7), w...

PFGE cpe Southern blot analyses of cpe-positive type A, C, D and E isolates.

Jihong Li (45207)
Kazuaki Miyamoto (219224)
Sameera Sayeed (338179)
Bruce A. McClane (45208)

February 2013

(A) DNA from type A, C, D or E isolates was subjected to PFGE prior to Southern blotting and hybr...

Genomic analysis of Clostridium botulinum group II by pulsed-field gel electrophoresis.

Hielm, S.
Björkroth, J.
Hyytiä, E.
Korkeala, H.

January 1998

Pulsed-field gel electrophoresis (PFGE) was optimized for genomic analyses of Clostridium botulinum ...

Southern blot showing hybridization of the probe for cje0215 of sequenced strain RM 1221 to DNA from isolates used in this study

Clifford G Clark (45469)
Lai-King Ng (32348)

December 2011

Pst I cut genomic DNA was hybridized and blotted as summarized in the Methods section. Sizes of the ...

Molucular gene profiling of Clostridium botulinum group III and their detection in naturally contaminated samples originating from various European countries

Woudstra, C.
LeMarechal, C.
Souillard, R.
Bäyon-Auboyer, M.H.
Anniballi, F.
Auricchio, B.
Medici, De, D.
Bano, L.
Koene, M.G.J.
Sansonetti, M.H.
Hansbauer, E.M.
Desoutter, D.
Dorner, M.B.
Fach, P.
Dorner, B.G.

January 2015

We report the development of real-time PCR assays for genotyping Clostridium botulinum group III tar...

C. botulinum strains analyzed in this study, and genomic location of their bont/B and /A PCR-RFLP subtype genes.

Giovanna Franciosa (348999)
Antonella Maugliani (373898)
Concetta Scalfaro (349002)
Paolo Aureli (349007)

February 2013

1CDC (Centers for Disease Control and Prevention, USA); ISS (Istituto Superiore di Sanità, Italy)...

Pulsed field gel electrophoresis (PFGE) and Southern blot hybridization results.

Sylvia Cardoso Leão (90815)
Cristianne Kayoko Matsumoto (336290)
Adriana Carneiro (104518)
Rommel Thiago Ramos (397218)
Christiane Lourenço Nogueira (397219)
James Daltro Lima Junior (397220)
Karla Valéria Lima (397221)
Maria Luiza Lopes (397222)
Horacio Schneider (287331)
Vasco Ariston Azevedo (397223)
Artur da Costa da Silva (397224)

April 2013

(A) PFGE-DraI and (B) Southern blot hybridization using the trfA-deri...

Distribution of bont/B PCR-RFLP subtype genes among 63 C. botulinum strains.

Giovanna Franciosa (348999)
Antonella Maugliani (373898)
Concetta Scalfaro (349002)
Paolo Aureli (349007)

February 2013

Distribution of bont/B PCR-RFLP subtype genes among 63 C. botulinum strains.</p

Plasmid-borne bont/B PCR-RFLP subtype genes among C. botulinum strains.

Giovanna Franciosa (348999)
Antonella Maugliani (373898)
Concetta Scalfaro (349002)
Paolo Aureli (349007)

February 2013

Plasmid-borne bont/B PCR-RFLP subtype genes among C. botulinum strains.</p

C.botulinum strains and sequences used in this study.

Piyada Wangroongsarb (515097)
Tomoko Kohda (515098)
Chutima Jittaprasartsin (515099)
Karun Suthivarakom (515100)
Thanitchi Kamthalang (515101)
Kaoru Umeda (515102)
Pathom Sawanpanyalert (147633)
Shunji Kozaki (515103)
Kazuyoshi Ikuta (114419)

January 2014

aThe subtypes and GenBank accession numbers for strains determined in this study are indicated in...

Comparison of DNA fingerprinting methods for use in investigation of type E botulism outbreaks in the Canadian arctic

Daniel Leclair
Franco Pagotto
Jeffrey M. Farber
Brigitte Cadieux
John W. Austin

January 2006

Pulsed-field gel electrophoresis (PFGE), randomly amplified polymorphic DNA (RAPD) analysis, and aut...

PFGE and hybridization analysis.

Marie Kempf (184731)
Jean-Marc Rolain (34775)
Georges Diatta (241918)
Saïd Azza (40837)
Bissoum Samb (318668)
Oleg Mediannikov (199078)
Amy Gassama Sow (318669)
Seydina M. Diene (318670)
Florence Fenollar (74934)
Didier Raoult (3833)

February 2013

A. Pulsed field gel electrophoresis (PFGE) profiles obtained for the two Acinetobacter ...

Confirmation of tagging C. botulinum BoNT-encoding plasmids pBotCDC-A3-Erm (strain CDC-A3), pCLJ-Erm (strain 657Ba) and pCLL-Erm (strain Eklund 17B) by PFGE and Southern hybridization analysis.

Kristin M. Marshall (364207)
Marite Bradshaw (364208)
Eric A. Johnson (225183)

February 2013

(B) Ethidium bromide stained PFGE of nondigested DNA samples from C. botulinum stra...

Confirmation of plasmid pBotCDC-A3-Erm transfer from C. botulinum strain CDC-A3580s1 to strain LNT01 by PFGE and Southern hybridization analysis.

Kristin M. Marshall (364207)
Marite Bradshaw (364208)
Eric A. Johnson (225183)

February 2013

(A) Ethidium bromide stained PFGE of C. botulinum DNA samples: SmaI digested...

Confirmation of plasmid pCLJ-Erm transfer from C. botulinum strain 657BaCT4 to strain LNT01.

Kristin M. Marshall (364207)
Marite Bradshaw (364208)
Eric A. Johnson (225183)

February 2013

(A) Ethidium bromide stained PFGE of C. botulinum strains: LNT01 (Lanes 1 and 7), w...

Confirmation of plasmid pCLL-Erm transfer from C. botulinum strain Eklund 17BCT11 to strain LNT01.

Kristin M. Marshall (364207)
Marite Bradshaw (364208)
Eric A. Johnson (225183)

February 2013

(A) Ethidium bromide stained PFGE of C. botulinum strains: LNT01 (Lanes 1 and 7), w...

PFGE cpe Southern blot analyses of cpe-positive type A, C, D and E isolates.

Jihong Li (45207)
Kazuaki Miyamoto (219224)
Sameera Sayeed (338179)
Bruce A. McClane (45208)

February 2013

(A) DNA from type A, C, D or E isolates was subjected to PFGE prior to Southern blotting and hybr...

Genomic analysis of Clostridium botulinum group II by pulsed-field gel electrophoresis.

Hielm, S.
Björkroth, J.
Hyytiä, E.
Korkeala, H.

January 1998

Pulsed-field gel electrophoresis (PFGE) was optimized for genomic analyses of Clostridium botulinum ...

Southern blot showing hybridization of the probe for cje0215 of sequenced strain RM 1221 to DNA from isolates used in this study

Clifford G Clark (45469)
Lai-King Ng (32348)

December 2011

Pst I cut genomic DNA was hybridized and blotted as summarized in the Methods section. Sizes of the ...

Molucular gene profiling of Clostridium botulinum group III and their detection in naturally contaminated samples originating from various European countries

Woudstra, C.
LeMarechal, C.
Souillard, R.
Bäyon-Auboyer, M.H.
Anniballi, F.
Auricchio, B.
Medici, De, D.
Bano, L.
Koene, M.G.J.
Sansonetti, M.H.
Hansbauer, E.M.
Desoutter, D.
Dorner, M.B.
Fach, P.
Dorner, B.G.

January 2015

We report the development of real-time PCR assays for genotyping Clostridium botulinum group III tar...

C. botulinum strains analyzed in this study, and genomic location of their bont/B and /A PCR-RFLP subtype genes.

Giovanna Franciosa (348999)
Antonella Maugliani (373898)
Concetta Scalfaro (349002)
Paolo Aureli (349007)

February 2013

1CDC (Centers for Disease Control and Prevention, USA); ISS (Istituto Superiore di Sanità, Italy)...

Pulsed field gel electrophoresis (PFGE) and Southern blot hybridization results.

Sylvia Cardoso Leão (90815)
Cristianne Kayoko Matsumoto (336290)
Adriana Carneiro (104518)
Rommel Thiago Ramos (397218)
Christiane Lourenço Nogueira (397219)
James Daltro Lima Junior (397220)
Karla Valéria Lima (397221)
Maria Luiza Lopes (397222)
Horacio Schneider (287331)
Vasco Ariston Azevedo (397223)
Artur da Costa da Silva (397224)

April 2013

(A) PFGE-DraI and (B) Southern blot hybridization using the trfA-deri...

Distribution of bont/B PCR-RFLP subtype genes among 63 C. botulinum strains.

Giovanna Franciosa (348999)
Antonella Maugliani (373898)
Concetta Scalfaro (349002)
Paolo Aureli (349007)

February 2013

Distribution of bont/B PCR-RFLP subtype genes among 63 C. botulinum strains.</p

Plasmid-borne bont/B PCR-RFLP subtype genes among C. botulinum strains.

Giovanna Franciosa (348999)
Antonella Maugliani (373898)
Concetta Scalfaro (349002)
Paolo Aureli (349007)

February 2013

Plasmid-borne bont/B PCR-RFLP subtype genes among C. botulinum strains.</p

C.botulinum strains and sequences used in this study.

Piyada Wangroongsarb (515097)
Tomoko Kohda (515098)
Chutima Jittaprasartsin (515099)
Karun Suthivarakom (515100)
Thanitchi Kamthalang (515101)
Kaoru Umeda (515102)
Pathom Sawanpanyalert (147633)
Shunji Kozaki (515103)
Kazuyoshi Ikuta (114419)

January 2014

aThe subtypes and GenBank accession numbers for strains determined in this study are indicated in...

Comparison of DNA fingerprinting methods for use in investigation of type E botulism outbreaks in the Canadian arctic

Daniel Leclair
Franco Pagotto
Jeffrey M. Farber
Brigitte Cadieux
John W. Austin

January 2006

Pulsed-field gel electrophoresis (PFGE), randomly amplified polymorphic DNA (RAPD) analysis, and aut...

PFGE and hybridization analysis.

Marie Kempf (184731)
Jean-Marc Rolain (34775)
Georges Diatta (241918)
Saïd Azza (40837)
Bissoum Samb (318668)
Oleg Mediannikov (199078)
Amy Gassama Sow (318669)
Seydina M. Diene (318670)
Florence Fenollar (74934)
Didier Raoult (3833)

February 2013

A. Pulsed field gel electrophoresis (PFGE) profiles obtained for the two Acinetobacter ...

PFGE and Southern blot hybridization of undigested bacterial DNA from the different <i>C. botulinum</i> strains and outbreak isolates to identify <i>boNT</i> gene location.

Abstract

Extracted data

PFGE and Southern blot hybridization of undigested bacterial DNA from the different <i>C. botulinum</i> strains and outbreak isolates to identify <i>boNT</i> gene location.

Abstract

Extracted data

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