Light- and gibberellin-related hypocotyl phenotypes of iPCC1 plants.

<p>Hypocotyl length of seedlings grown under (A) 10 µmole m⁻² s⁻¹ of red light (RL), 30 µmole m⁻² s⁻¹ of blue light (BL), or 5 µmole m⁻² s⁻¹ of far-red light (FRL) for 4 days, and (D) the same light conditions as in (A) but treated as indicated with the gibberellin synthesis inhibitor paclobutrazol (PAC). (B) <i>PCC1</i> transcript levels were quantified by qRT-PCR in wild type Col-0 and the indicated photoreceptor mutant seedlings. Values are the mean of three independent biological replicates ± SD and are expressed as relative levels to those detected in wild type seedlings. (C) Hypocotyl length of seedlings grown under 60 µmole m⁻² s⁻¹ of white light (WL), with the indicated µM concentrations of GA3 (GA). Values are the mean of 20 hypocotyls per genotype and condition ± SD. * and ** represents statistically significant (p<0.05 or p<0.01, respectively, in Students t-test) different values in iPCC1 seedlings when compared to wild type seedlings under the same condition. (E) Transcript levels of the indicated genes involved in the perception and signaling of gibberellins were analyzed by qRT-PCR in Col-0 and iPCC1 seedlings grown under 16 h light/8 h darkness photoperiodic white light conditions for 14 days. Values are the mean of three independent biological replicates ± SD and are expressed as relative levels to those detected in wild type seedlings. * represents statistically significant (p<0.05 in Students t-test) different values in iPCC1 seedlings when compared to wild type seedlings.