Deletion B₁R promoter constructs in HIGH expressing H2126 and LOW expressing 16HBE reveal regulatory regions.

<p>Summary of results from deletion constructs of B₁R regulatory regions (A). The size of each regulatory region is indicated relative to start of exon III of B₁R (+1). PRE = positive regulatory element, NRE = negative regulatory element. Relative luciferase activity of promoter deletion constructs transfected into human lung adenocarcinoma H2126 (B) and human bronchial epithelium 16HBE (C). Data presented as mean with error bars representing SEM. Data was analysed using one-way ANOVA and Tukey's post-hoc test on 4 independent experiments, each performed using at least triplicates. *p≤0.05 was considered statistically significant. Activity of CP-E2I2-Luc construct containing the B₁R 5′ promoter and −1020 bp to +1 was set to 1.